Beilstein J. Org. Chem.2022,18, 567–579, doi:10.3762/bjoc.18.59
reasons, the synthesis of N-acetylneuraminic acid (Neu5Ac) in a continuousfixed-bedreactor by an immobilized epimerase and aldolase was investigated in detail. The immobilized enzymes showed high stability, with half-life times > 173 days under storage conditions (6 °C in buffer) and reusability over 50
for the aldolase from 108 to 42 mM and 91 to 37 mM, respectively.
Keywords: aldolase; continuousfixed-bedreactor; enzyme; epimerase; GlcNAc; high pressure; immobilization; ManNAc; Neu5Ac; pyruvate; Introduction
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Graphical Abstract
Figure 1:
Reaction sequence starting from GlcNAc with ManNAc as an intermediate. Pyr is added in the second s...